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KMID : 0352819890050010019
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Kosin Medical Journal
1989 Volume.5 No. 1 p.19 ~ p.25
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Histochemical Study of Effects of Arsenic on Na^(+) -K^(+) -Activated Adenosine Triphosphatase Activity in Tubular Epithelial Cells of Rat Kidney
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Abstract
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1
Arsenic, especially trivalent form, produces highly toxic effects to living organisms. By binding to sulfhydryl groups in proteins, arsenci interferes with many enzymes essential to cellular metabolism. In this study, histochemical changes in ATPase activity in the rat kidney induced by arsenic were investigated.
Male adult rats(Sprague-Dawley strain) were treated with arsenic(As₂O₃)in three different doses, 10, 13 and 16㎎/㎏ body weight. The changes in the tubular epithelial cells of the kidney were investigated by enzyme histochemical methods.
With H-E and PAS staining, the control and experimental groups did not show appreciable difference in morphology.
With ATPase reaction, the epithelial cells of the distal tubules of the control group exhibited stronger enzymatic reaction than the cells of the proximal tubules. In the arsenic-treated rats, the proximal and distal tubules showed decreased enzymatic activity, especially in the latter.
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